Gel isoelectric focusing of cerebrospinal fluid proteins: a potential diagnostic tool.
نویسنده
چکیده
Isoelectric focusing of proteins is now well established as a powerful tool in protein chemistry following its introduction a few years ago (1, 2). Unfortunately, this new technique has not yet found practical day to day use in the·, clinical laboratory. This communication describes a reproducible and easy method for gel isoelectric focusing (3, 4, 5)! It also contains the preliminary' results obtained with cerebrospinal fluid proteins, mostly from multiple sclerosis cases.
منابع مشابه
Analysis for cerebrospinal fluid proteins by isoelectric focusing on polyacrylamide gel: methodological aspects and normal values, with special reference to the alkaline region.
lsoelectric focusing on polyacrylamide gel is an electrophoretic separation method with high resolution capacity. This makes it excellent for research work, but difficult to use in routine diagnostic work, as it may be difficult to distinguish between normal and pathological protein patterns. Methods such as electrophoresis on agar or agarose gels with a lower resolution capacity seem to be eas...
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ورودعنوان ژورنال:
- Zeitschrift fur klinische Chemie und klinische Biochemie
دوره 9 4 شماره
صفحات -
تاریخ انتشار 1971